Anti-inflammatory effect of external use of escin on

ZHAO Shu-Qi , XU Shi-Qiang , CHENG Jing , CAO Xiao-Lu , ZHANG Ying , ZHOU Wei-Ping , HUANG Yan-Juan , WANG Jun  , HU Xia-Min

[ABSTRACT] Escin, as an internally applied anti-inflammatory agent, has been widely used in the treatment of inflammation and edema resulting from trauma or operation in the clinic. However, the effect of its external use on cutaneous inflammation and edema remains unexplored. In the present study, the anti-inflammatory and anti-edematous effects of external use of escin were studied in carrageenan-induced paw edema and histamine-induced capillary permeability in rats, paraxylene-induced ear swelling in mice, and cotton pellet-induced granuloma in rats. Effects of external use of escin gel on prostaglandin E2 (PGE2), tumor necrosis factor-α (TNF-α), and interleukin-1β (IL-1β) were determined by ELISA ...

[ELISA for PGE2, TNF-α, and IL-1β analyses] The levels of PGE2, TNF-α and IL-1β were determined according to LI’s method  [1] . In brief, the tissue samples were obtained from paw edema and capillary permeability models,followed by homogenizing with ice-cold saline for a 10%(W/V) homogenate. The ELISA kits of PGE2 (JianglaiBio, Shanghai, China[1]), TNF-α (Lianke Bio, Wuhan, China) and IL-1β (Lianke Bio, Wuhan, China) were use, according to manufacturer’s instructions.

[備注] [1]“江萊生物”10年以來一直致力于免疫學(xué)技術(shù)的積累與沉淀，目前江萊生物實驗室占地約3000平米，技術(shù)研發(fā)團隊50余人，配備了全球最先進的分析和檢測設(shè)備，我們通過自主創(chuàng)新以及與國內(nèi)外的科研單位合作研發(fā)，不斷突破新技術(shù)，目前我們研發(fā)了上千種指標(biāo)，質(zhì)量成熟，并長期出口到歐美市場，贏得了眾多科研機構(gòu)的認(rèn)可 ...

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