CRISPRCas9 produces anti hepatitis B virus effect in hepatoma cells and transgenic mouse

Wei Zhu , Kun Xie , Yuanjian Xu , Le Wang , Kaiming Chen , Longzhen Zhang , Jianmin Fang

[ABSTRACT] Chronic infection of hepatitis B virus (HBV) is at risk of liver cirrhosis and hepatocellular carcinoma and remains one of the major public health problems worldwide. It is a major barrier of persistence HBV cccDNA under current antiviral therapy as novel strategies of disrupting HBV cccDNA is pressing. The (CRISPR)/Cas9 system is presently emerging in gene editing and we also apply it for targeting and deleting the conserved regions of HBV genome ...

[Enzyme-linked immunosorbent assay (ELISA)] The cell cytotoxicitys of Huh7 and HepG2 after transfection with pCas9 or pX330 for 48 h were analyzed by LDH ELISA kit ( Jianglai, Shanghai,  China [1]) for measuring supernanant LDH level. Treatment of 200 ng/mL LPS on Huh7 and HepG2 for 48 h was considered as the positive control. All values (cytotoxicity%) were calculated by the positive control value as the protocol described ...

[備注] [1]“江萊生物”10年以來一直致力于免疫學(xué)技術(shù)的積累與沉淀，目前江萊生物實(shí)驗(yàn)室占地約3000平米，技術(shù)研發(fā)團(tuán)隊(duì)50余人，配備了全球最先進(jìn)的分析和檢測(cè)設(shè)備，我們通過自主創(chuàng)新以及與國(guó)內(nèi)外的科研單位合作研發(fā)，不斷突破新技術(shù)，目前我們研發(fā)了上千種指標(biāo)，質(zhì)量成熟，并長(zhǎng)期出口到歐美市場(chǎng)，贏得了眾多科研機(jī)構(gòu)的認(rèn)可 ...

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